Flow Cytometry Stem Cell Enumeration

The advent and use of a cd34 monoclonal antibody enabled the detection of these cells by flow cytometry.

Flow cytometry stem cell enumeration.

Furthermore cd34 cell enumeration is useful for monitoring the stem cell yield during graft manipulations such as immunomagnetic selections ex vivo expansion or genetic modifications of hscs. Flow cytometric enumeration of cd34 cells is commonly employed to assess the hematopoietic stem cell hsc numbers in peripheral blood cord blood and apheresis products used for peripheral blood stem cell pbsc transplantation. Enumeration of cd34 cells in cord blood. I am a development technologist for the human cellular therapy laboratory at mayo clinic.

Detection of minimal residual disease. The cd34 antigen is stage specific and identifies cells in the early stages of hematopoietic differentiation. A variation on a single platform flow cytometric method based on the ishage gating strategy. Issues in enumeration applications of flow cytometry in stem cell research and tissue regeneration 10 1002 9780470631119 115 134 2012.

Hematopoietic progenitor cells or hpcs are used to reconstitute hematopoiesis following marrow ablative therapies. For the analysis of cd34 hscs cell samples are stained with fluorescently labeled antibodies against both the hsc antigen cd34 and the pan leukocyte antigen cd45. Identification of cd34 hematopoietic stem cells cd34 cell enumeration by flow cytometry is a rare event analysis. Centers for disease control.

Bd stem cell enumeration starter kit includes software for first time users. Flow cytometric enumeration of cd34 hematopoietic stem and progenitor cells hscs is widely used for evaluation of graft adequacy of peripheral blood and bone marrow stem cell grafts. Immunophenotyping of blood products. Cytokine and chemokine profiling.

Robert sutherland hematopoietic stem cells. Multicolor flow cytometry stem cell research t cell research support support sales and support faq technical support training resources and tools. Size 50 tests status ivd cat no 656296. Several flow cytometric studies have shown.